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Implementing ‘mix and rotate’ strategy to combat herbicide resistance

with Tony Lockrey, consulting agronomist, AMPS Moree

Mixing and rotating herbicide modes of action is a key strategy in the WeedSmart Big 6 – but it’s a herbicide response to a herbicide problem. So, while it’s critical, it must be implemented within a diverse weed management program.

Tony Lockrey, senior agronomist with AMPS Agribusiness at Moree has seen herbicide resistance get out of control on some farms in northern NSW while other growers have responded early and managed to maintain a broader spectrum of effective herbicides in their program.

AMPS Moree consulting agronomist Tony Lockrey has seen good results when herbicides are rotated and mixed in each phase – the fallow, pre-sowing, in-crop and for desiccation.

“It has to start with herbicide resistance testing – specifically for susceptibility,” he says. “Knowing what does work is very important as you’ve probably already got a fair idea about what doesn’t.”

Once all the effective actives are ‘on the table’ it’s time to look at what crops can be grown to allow the use of the widest range of herbicide groups in the rotation, and where you might be able to find synergistic mixes that can further delay resistance and potentially allow the use of actives that are no longer effective on their own.

“When we sit down to plan out an integrated weed control program we want to make sure there is rotation and mixing going on in each phase – in the fallow, pre-sowing, in-crop and for desiccation, where required,” says Tony. “When this is done in conjunction with a determination to stop seed set and remove survivors then it is possible to keep weed numbers low.”

With an increasing number of proprietary herbicide mixes coming onto the market and the broad spectrum of synergistic and antagonistic interactions between potential mixing partners it pays to be well-informed and to seek advice.

If I already rotate modes of action why do I have to mix too?

Short answer: Rotation buys you time; mixing buys you shots. Mixing and rotating buys you time and shots.

Longer answer: Rotation of effective modes of action can significantly delay the onset of herbicide resistance and needs to be built into your crop rotation plan. Herbicides in Group A and Group B are particularly susceptible to multiple exposure resistance with as few as six exposures being enough to select for the resistant mutation.

By mixing MOA groups, either in the same tank mix or applied separately to the same population (like a double knock), those plants that survive one MOA are often killed by the second.

How does testing for susceptibility help when there’s a weed blow-out?

Short answer: Knowing what will work against a resistant population helps drive down the seed bank and helps you regain control.

Longer answer: One real-world example is a paddock near Moree where Group A resistant wild oats were discovered in 1998 following a history of repeated use of Topik® (Group A – fop), Verdict® (Group A – fop) and, later, Axial® (Group A – den). Testing of this population showed the wild oats was very susceptible to Group B sulfonylurea, so Atlantis was used to drive down the weed numbers. A new plan was then put in place with Groups B, A, C and M used across the winter cropping program, but there was still too much reliance on Group B. The current plan for the farm now includes pre-emergent herbicides from Groups K, J and D used individually and in mixes.

How do I integrate more mixes into my herbicide program?

Short answer: Look for opportunities for synergistic mixes throughout the fallow and cropping seasons. In many instances the most important mixing partner is more water.

Longer answer:

Many growers are looking for tank mixes to improve control of glyphosate-resistant seedlings. Knowing which mixtures are beneficial and which are antagonistic is important.

In the fallow, there are often opportunities to use the mix and rotate strategy to great effect in a double-knock application, such as:

  • Group M (glyphosate) + Group I (2,4-D or fluroxypyr or picloram) followed by Group L (paraquat)
  • Group M (glyphosate) followed by Group L (paraquat) + Group G (Sharpen® or flumioxazin)
  • Group M (glyphosate) followed by Group L (paraquat) + Group K (Dual® Gold)
  • Group A (Shogun®) followed by Group L (paraquat) + Group K (Dual® Gold)

The fleabane on the right was unresponsive to glyphosate on its own but mixing picloram with triclopyr or 2,4 D to the glyphosate application was effective (left). 

Pre-plant examples include paraquat plus a triazine herbicide (Group C) or paraquat plus an imidazalinone (Group B), which are commonly used to provide broad spectrum knockdown and residual control. Dual® Gold (Group K) is another common fallow residual option which is very compatible with glyphosate, triazines and paraquat.

An example of an in-crop mix is the addition of clethodim to haloxyfop (both Group A) to improve control of fop-resistant grasses in broadleaf crops where both are registered.

At the end of the season there is also some opportunity to mix desiccants for some crops.

None of these mixes are provided as recommendations – seek advice for your own situation and always read and follow the label.

What about application set up for mixtures?

Short answer: Some herbicides require better coverage. In many instances the most important mixing partner is more water.

Longer answer: Suitable product and water rates, droplet size and the right adjuvant, are critical for optimising herbicide efficacy.

For example, while a fallow mix such as glyphosate plus a Group A, or a Group G (depending on the target weed), is physically compatible, the components have different requirements for optimal performance. Seek advice about the best water rate to use, the potential impact of an oil-based adjuvant (required for most Group A and Group G herbicides) on glyphosate efficacy for some summer grass weeds, and other possible risks.

Factsheet – Mixing knockdown partners with Group G

How do I avoid generating multiple and cross-resistance?

Short answer: Implement as many different weed control strategies as possible. The WeedSmart Big 6 is a practical foundation for an integrated program of herbicide and non-herbicide tactics.

Longer answer: Rotating and mixing herbicide groups can give you room to move in holding off resistance or getting more out of some marginally effective products.

The only way to stave off herbicide resistance completely is to have low weed numbers and to be vigilant about preventing survivors from setting seed. Have a diverse cropping program, use herbicides to provide early weed control, set your crops up to compete strongly and monitor and remove survivor weeds.

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What can I do to control large FTR grass in fallow?

Feathertop Rhodes grass (FTR) is a major weed in chemical fallows in Australia, and is notoriously hard to kill with glyphosate.
Bhagirath Chauhan, professor at the University of Queensland’s Centre for Crop Science, says some other herbicide control measures have potential to manage large FTR plants (40 to 50 leaf stage) that have escaped earlier treatment.
Professor Bhagirath Chauhan says there are some tank mixes and herbicide sequences that growers could deploy to help manage FTR and stop seed set.
“Feathertop Rhodes grass is an aggressive weed that can establish in bare fallow situations and produce a large quantitiy of seed if left uncontrolled,” he says. “Several biotypes of this species are resistant to glyphosate and can also survive a double knock of glyphosate followed by paraquat, particularly once the weed is larger than 4 to 5 leaf stage.”
To give growers more options, a study was conducted to assess the potential of other herbicides and use patterns that can control large feathertop Rhodes plants or stop seed set.   
Alternative herbicide options are available to help manage large FTR and reduce seed set in fallow.
“An integrated approach is essential to controlling feathertop Rhodes grass,” says Bhagirath. “In applying the WeedSmart Big 6 to FTR in a bare fallow situation we have identified some tank mix and herbicide sequences that growers could deploy to help manage this difficult weed and stop seed set.”
Can anything be done to improve the efficacy of glyphosate or the double knock against large FTR plants?
In brief: Adjuvants did not improve glyphosate efficacy on mature (40 to 50 leaf) FTR plants. In glyphosate resistant populations, the second knock product is doing the heavy lifting when applied to large (8 to 10 leaf) FTR plants.
The details: None of the commercially available adjuvants improved the efficacy of glyphosate (740 g a.e. per ha) as a single product application on FTR at the 40 to 50 leaf stage. All the plants survived and produced seed after being treated with glyphosate, indicating that the population used in the study was resistant to glyphosate at this rate and weed growth stage.
Glyphosate and the double knock tactic can often provide good control of resistant FTR plants if the herbicide is applied when the plants are small and actively growing.
The traditional double knock of glyphosate (Group 9 [M]) or glyphosate + 2,4-D, followed by paraquat (Group 22 [L]) or glufosinate (Group 10 [N]), applied to older FTR plants (8-10 leaf) achieved increased phytotoxicity through improved mortality, reduced biomass or fewer seed panicles.
However, the double knock was no better than using paraquat or glufosinate alone when applied to 8 to 10 leaf FTR plants. FTR is not listed on glufosinate labels in Australia but is used to control other weeds in fallow situations at the rate (750 g a.i. per ha) tested in this study. For best results, glufosinate needs to be applied in warm, humid conditions, which is not a common scenario for summer fallow situations.
Rate response (0, 187.5, 375 and 750 g a.i. per ha) to glufosinate applied to large FTR plants.
Are clethodim or haloxyfop suitable alternative herbicides to treat large, glyphosate resistant FTR plants?
In brief: Possibly. Excellent results were achieved in pot trials conducted in an open environment, but will be more difficult to achieve in the field.
The details: Clethodim and haloxyfop were tested on FTR plants at the 24 to 28 leaf stage. Clethodim is registered for use against FTR in a number of summer crops, but without any crop competition many FTR plants survived the registered rate (90 g a.i. per ha), although weed biomass and seed production was severely curtailed.
Haloxyfop efficacy against FTR at this growth stage was 100 per cent at the registered rate of 80 g a.i. per ha.
A combination of these two treatments also resulted in 100 per cent control. The effective use of these two herbicides (both Group 1 [A]) relies on excellent coverage and application when the plants are actively growing. This is difficult to achieve in field conditions, which is why the label recommendations are typically for younger weeds.
A combination of clethodim and haloxyfop can provide good control of large feathertop Rhodes plant and curtail seed production.
These herbicides are known to readily select for resistant biotypes so when applied in a chemical fallow situation (with no competition), it is necessary to target small weeds with robust application rates and to apply a second knock with a contact herbicide, such as paraquat. 
Did you find any new and exciting prospects for controlling mature FTR plants?
In brief: Yes, it seems that there is a truly synergistic effect when isoxaflutole (Group 27 [H]; e.g. Balance) is mixed with paraquat.
The details: Neither of these herbicides provided useful control of FTR at the 40 to 50 leaf stage when applied individually. When mixed together, these herbicides achieved a higher level of weed mortality and prevented panicle production. For example, a tank mixture of isoxaflutole 75 g a.i. per ha, with paraquat 600 g a.i. per ha, resulted in 92 per cent FTR mortality and no panicle production.
Even at a paraquat rate of 300 g a.i. per ha mixed with isoxaflutole 75 g a.i. per ha, only 17 per cent of the large FTR plants survived when the mixture was applied to both the plant and the nearby soil – allowing uptake through both the leaves and the roots.
The benefit of this mixture may be reduced if the weed patch is dense, potentially reducing the amount of the isoxaflutole that reaches the soil. Even the prevention of seed set in large FTR plants is of significant value in managing the seed bank of this invasive weed, as FTR seed remains viable for less than 12 months.
Such a use pattern is not currently specified on product labels, although both products are registered for weed control in fallow situations.  
Web resources
Read the research paper.

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News

Testing informs herbicide choice

Buying a new property brings with it a number of unknowns and it can take some time for the new owners to become familiar with the soils, the terrain and the weeds.
When the Birch family of ‘Catalina Farms’, Coorow, WA purchased a neighbouring property, historic ‘Koobabbie’, they had reason to expect that herbicide resistance would be less of a problem because there had been limited use of herbicides on ‘Koobabbie’ in its 114 history of farming.
Daniel Birch, who farms with his wife Jen, parents Rod and Shelley, and long-term team member, Justin Passamani, says the purchase conveniently coincided with the opportunity to be involved with independent herbicide resistance testing in 2020.
“The testing was arranged through the Liebe Group and conducted at the Australian Herbicide Resistance Initiative,” he says. “We were keen to test some theories we had about resistance status of weeds on Catalina and also to get some baseline information about Koobabbie to help us plan our herbicide program.”
Daniel Birch of ‘Catalina Farms’, Coorow, WA says the big take home message from recent herbicide resistance testing was the power of mode of action mixtures.
The testing revealed a number of things that surprised the Birches. To start with, they found there was Group 2 [B] resistant ryegrass in paddocks on Koobabbie that had no history of Group 2 herbicide use.
“This demonstrated just how easy it is for herbicide resistance to move in seed or hay, or on machinery,” says Daniel. “Obviously we can use this information to avoid using chemistry that we know has little to no efficacy on ryegrass.”
“The other major finding was that Group 12 [F] resistance in wild radish was much higher than we expected across the farm,” he says.
This has led the Birches to include more premium products in their program to target resistant ryegrass and wild radish, and drive down the weed seed bank as quickly as possible. On the flip side, they also discovered that they can save money by using trifluralin at lower rates in seasons with good growing conditions, where there is less need for a long residual effect.
“In those years where the crop gets off to a good start, the crop competition effect kicks in early to suppress weeds,” says Daniel.
By taking on board the resistance testing results from weed seed samples collected across the state, Daniel was reassured by the fact that the resistance issues they faced were essentially the same as other growers.
“The big take home message for us was the power of mode of action mixtures,” he says. “From the overall survey results across Australia, the resistance frequency to stand-alone pre-emergence herbicides ranged from 10 to 34 per cent, yet resistance to herbicide mixtures ranged from 0 to 6 per cent.”
Armed with knowledge about the resistance profile of their weeds, the Birches are combining some older chemistry with newer, premium herbicides for maximum effect.
The AHRI resistance testing program is led by Dr Roberto Busi. In 2019, annual ryegrass seed samples from 298 farms were submitted, representing 579 populations from four states in Australia, and these were tested for resistance to 21 herbicides applied at the recommended rate – 12 standalone and nine two-way mixtures.
In total, 15 876 individual resistance tests were conducted to screen two million seeds against registered herbicides and herbicide mixtures at the recommended label rate.
Dr Busi says the mixtures that growers can confidently incorporate in their annual ryegrass program are trifluralin + Sakura mix, Luximax + triallate, and clethodim + butroxydim.
“When applied at full rate for each component, these mixtures can achieve a better outcome than the same herbicides applied as stand-alone treatments against annual ryegrass with known resistance,” he says.
For wild radish, 200 samples were tested over a period of two years and resistance to Groups 2 [B], 4 [I] and 12 [F] were all over 50 per cent resistant. At 70 per cent resistant, Group 2 [B] herbicides should probably be dropped from most wild radish herbicide programs and Group 4 [I] is under threat.
“In 2021, we found that mixing Group 12 [F] with Group 6 [C] herbicide bromoxynil vastly improved control of wild radish,” he says. “From 51 per cent resistant to Group 12 [F] down to less than 15 per cent of samples resistant to the 12 [F] plus 6 [C] mixture.”
“It is important to emphasise that herbicide resistance testing is conducted on small, actively growing weed seedlings in a glasshouse environment,” says Roberto. “In the field, spray failures can easily occur, even in susceptible weed populations, if the herbicide is applied under the wrong conditions or to plants that are too large or stressed. This is particularly true for wild radish.”
AHRI Podcast: Interview with Daniel Birch about herbicide testing
AHRI Insight: Mixtures rock
Herbicide testing options

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News

Safe sorghum planting while controlling weeds

Pre-emergent herbicide, metolacholor and S-metolachlor, have been used in Australia primarily to control grass weeds for over 40 years. To date, no instances of resistance to this active ingredient have been documented in Australia and even internationally the few cases of resistance are in broadleaf weeds only. Resistance in northern summer grasses is probably low at present.
Syngenta’s field biology manager, Rob Battaglia, says some recent changes to the label use patterns and the launch of a new seed safener product will further expand the usefulness of S-metolachlor products, such as Dual Gold® and Primextra Gold®, in summer crops while protecting sorghum seedlings from herbicide damage.
Syngenta’s field biology manager, Rob Battaglia, says the new use patterns for Dual Gold® in sorghum, cotton and fallow centre on extending application flexibility and residual activity of the herbicide on target weeds.
“S-metolachlor is registered for use in a wide range of crops, including sorghum, maize, sweetcorn, soybean, sunflower and cotton, as well as in fallow situations,” he says. “The new use patterns in sorghum, cotton and fallow centre on extending application flexibility and residual activity of the herbicide on target weeds.”
In sorghum, a rate of 1.0 to 2.0 L/ha can be applied either as a single application before the crop or weeds emerge, or as a split application pre and post crop emergence (up to 6-leaf stage). Similarly, the full rate can be applied after harvest to establish the fallow or 1.0 to 1.5 L/ha can be applied pre-emergent to weeds at fallow establishment followed by 0.5 to 1.0 L/ha within 4 weeks. Be sure to observe replant intervals when planning the next crop.
In cotton, a single application of 1 L/ha either pre-emergent (before, at, or immediately after, planting), over-the-top or directed stand-alone spray or over-the-top mixed with Roundup Ready herbicide on Roundup Ready FLEX® cotton.
“There are several factors that affect the length of effective residual activity of S-metolachlor, some of which are outside the grower’s control,” says Rob. “The product needs to be incorporated and has interactions with rainfall, temperature, soil type, soil organic matter and stubble. Having more flexibility in the application timing gives growers the ability to compensate for some of these factors and control new waves of weed germinations for longer.”
Untreated strips are a good indicator of the seed bank and potential weed burden in a field. The pre-emergent herbicide treated area on the left has far fewer weeds than the untreated section of the field on the right, which suffered significant yield loss due to feathertop Rhodes grass infestation.
S-metolachlor is only active on weeds that have not yet emerged and has no effect on weeds that have already emerged from the soil or that do not come in contact with the herbicide as they emerge from the soil. It is essential to control already-emerged weeds first and then apply the pre-emergent to control subsequent flushes of weed germinations.
“There are some situations where the residual herbicides will appear to be less effective than they should, but this can often be attributed to compromised application timing, limited rainfall and soil conditions, rather than herbicide performance,” says Rob. “The difficultly in achieving perfect application timing and incorporation with rainfall is one of the main reasons why the new use patterns for Dual Gold® have been registered. Pre-emergent herbicides are best used within the WeedSmart Big 6 integrated weed management program and not as a stand-alone control measure.”
While other crops listed on the S-metolachlor label can metabolise the herbicide, sorghum requires a seed safener to protect the germinating plants from the herbicidal effects of S-metolachlor. The new Epivio C® seed safener replaces Concept II® and provides better overall protection for sorghum seed.
S-metolachlor damage is seen as distorted and twisted growth in sorghum, the rightmost plant is unaffected (protected by seed safener).
In Syngenta’s product development trials in Queensland and NSW, they measured an 11 per cent increase in plant stand and a 7 per cent increase in sorghum yield in crops where the seed was treated with Epivio C®compared to Concept II®. Epivio C® has also demonstrated improvements in seed safety and shelf-life for carry-over seed.
“Epivio C® is applied as seed treatment and when the seed is planted into moist soil the product is taken up into the plant and improves the metabolism of the herbicide in the seedling, resulting in no crop symptoms,” says Rob. “The result is better crop establishment and healthier plants that can better compete against weeds that germinate later in the season.”
Epivio C plot trial demonstrating the benefit of the seed safener in supporting robust germination and establishment. No safener (left) compared to Epivio C safener (right).
There is evidence that growers can maintain crop yield and reduce summer grass seed production by planting sorghum crops at a density of 10 plants/m2 and a row spacing of 50 cm.
The WeedSmart Big 6 includes diversity in crops and pastures, crop competition and mixing and rotating herbicide mode of action groups.
More information

Podcast: Seed safener explainer (starts around the 22 min mark) 
Article: Weaponise sorghum crops to take out feathertop Rhodes grass and awnless barnyard grass

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