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Stewardship of synthetic auxin herbicides

with Roberto Busi, Research Fellow, AHRI

Globally, resistance to the world’s oldest herbicide is relatively rare. Unfortunately, one of the economically significant examples of auxin (2,4-D, dicamba and MCPA) resistance is in wild radish populations in Western Australia.

Roberto Busi, research fellow with the Australian Herbicide Resistance Initiative (AHRI), says the recent and future release of new crops with resistance to synthetic auxins (USA only) will heighten the risk unless stewardship measures are followed.

Dr Roberto Busi, AHRI research fellow says researchers around the world are working to better understand the way auxin herbicides work and the mechanisms plants use to evolve resistance.

“The synthetic auxin herbicides have been in wide-spread use since their discovery in the 1940s. According to a 2014 Dow AgroSciences report, herbicides such as 2,4-D and dicamba are used on about 200 million ha world-wide. They mimic the activity of natural plant hormones and seem to have multiple sites of action along with both physiological and biochemical effects that lead to impaired plant growth and death,” he says. “The rate of resistance evolution has been lower than other herbicide modes of action.”

“2,4-D resistant wild radish was first documented in Western Australia in 1999,” says Dr Busi. “Since then surveys have revealed some populations with high-level 2,4-D resistance and a total of 60 per cent of randomly surveyed samples in 2003 that has levelled out at around 74 per cent in both 2010 and 2015.”

Multiple resistance to at least two herbicides is quite common in wild radish populations in Western Australia, generally to chlorsulfuron and 2,4-D, however the last survey also showed alarming levels of resistance to diflufenican. However, resistance to atrazine, glyphosate or Group H herbicides remains rare or is not reported.

“Western Australian farmers have been able to find other ways to reduce the overall number of wild radish plants in their crops by running down the soil seed-bank and this is the correct way to overcome resistance,” says Dr Busi. “If auxin herbicide use were to increase or be used more frequently in Australian cropping rotations growers would need to implement non-herbicidal strategies that reduce the risk of herbicide resistance.”

Group I resistance has also been recorded in wild radish in South Australia, Victoria and New South Wales and in other species including Indian hedge mustard, sowthistle and capeweed.

Why did wild radish in WA evolve 2,4-D resistance when it is so rare globally?

Short answer: Wild radish in the northern WA wheatbelt infested paddocks at high density and the repeated use of Group B and 2,4-D rapidly selected for high levels of resistance.

Longer answer: Growers and agronomists are now well aware that simply swapping from one herbicide to another does not help manage herbicide resistance. This lesson was learnt the hard way in WA where 2,4-D initially provided excellent control of Group B-resistant wild radish. Failure ensued because 2,4-D was applied frequently to dense populations of wild radish, a species that is a prolific seeder and the seed can persist in the soil for many years. In two study populations we found that 2,4-D resistance was clearly inherited as a single, nuclear (pollen transmitted) dominant or near-dominant gene trait. This contributed to the speed of resistance evolution to 2,4-D in wild radish. In other species the traits conferring 2,4-D resistance may be less frequent or be less genetically dominant, and that slows the evolution of resistance.

Are 2,4-D resistant plants less fit than susceptible plants?

Short answer: Following a 2,4-D application, surviving weeds are less competitive.

Longer answer: True fitness penalty has not been established for 2,4-D resistant weeds. A ‘fitness penalty’ is when a resistant plant, growing in the absence of the herbicide is less fit than a susceptible plant.

AHRI research has demonstrated that when 2,4-D resistant plants are sprayed with 2,4-D they are suppressed, show strong symptoms of herbicide damage, but do not die. However, if these plants are sprayed when they are small and grow in competition with a crop, the resistant weeds may die or may produce far less seed than it would in a non-competitive situation.

Crop competition can therefore be used as a weapon against 2,4-D resistant weeds while continuing to use 2,4-D or MCPA as part of a tank mix.

The release of new auxin herbicide formulations in Australia means farmers have more options for broadleaf weed control but researchers emphasise the heightened need for stewardship to reduce the risk of resistance and the importance of crop competition in managing resistance.

How can tank mixes extend the use of herbicides that weeds are resistant to?

Short answer: Multiple resistance to auxin herbicides and other MOA is rare in individual plants. Focus on low seed-banks, use herbicide mixes and mix it up with non-herbicide strategies to keep ahead of resistance.

Longer answer: The most important objective is to aim for low weed numbers. In this situation, while a population of wild radish in a paddock may be resistant to a number of herbicide modes of action the chance of an individual plant being resistant to more than one MOA is very low. In plants such as wild radish that are cross-pollinated there is a lot of mixing of the genetic material so stacking of resistant traits is initially uncommon, particularly for resistance traits that are recessive. Consequently, if a tank mix of three compatible herbicides with different modes of action is applied, with each herbicide at robust label rates, an individual plant is highly likely to be susceptible to at least one of the herbicides applied and so overall plant numbers are reduced through the use of the tank mix.

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What can I do to control large FTR grass in fallow?

Feathertop Rhodes grass (FTR) is a major weed in chemical fallows in Australia, and is notoriously hard to kill with glyphosate.
Bhagirath Chauhan, professor at the University of Queensland’s Centre for Crop Science, says some other herbicide control measures have potential to manage large FTR plants (40 to 50 leaf stage) that have escaped earlier treatment.
Professor Bhagirath Chauhan says there are some tank mixes and herbicide sequences that growers could deploy to help manage FTR and stop seed set.
“Feathertop Rhodes grass is an aggressive weed that can establish in bare fallow situations and produce a large quantitiy of seed if left uncontrolled,” he says. “Several biotypes of this species are resistant to glyphosate and can also survive a double knock of glyphosate followed by paraquat, particularly once the weed is larger than 4 to 5 leaf stage.”
To give growers more options, a study was conducted to assess the potential of other herbicides and use patterns that can control large feathertop Rhodes plants or stop seed set.   
Alternative herbicide options are available to help manage large FTR and reduce seed set in fallow.
“An integrated approach is essential to controlling feathertop Rhodes grass,” says Bhagirath. “In applying the WeedSmart Big 6 to FTR in a bare fallow situation we have identified some tank mix and herbicide sequences that growers could deploy to help manage this difficult weed and stop seed set.”
Can anything be done to improve the efficacy of glyphosate or the double knock against large FTR plants?
In brief: Adjuvants did not improve glyphosate efficacy on mature (40 to 50 leaf) FTR plants. In glyphosate resistant populations, the second knock product is doing the heavy lifting when applied to large (8 to 10 leaf) FTR plants.
The details: None of the commercially available adjuvants improved the efficacy of glyphosate (740 g a.e. per ha) as a single product application on FTR at the 40 to 50 leaf stage. All the plants survived and produced seed after being treated with glyphosate, indicating that the population used in the study was resistant to glyphosate at this rate and weed growth stage.
Glyphosate and the double knock tactic can often provide good control of resistant FTR plants if the herbicide is applied when the plants are small and actively growing.
The traditional double knock of glyphosate (Group 9 [M]) or glyphosate + 2,4-D, followed by paraquat (Group 22 [L]) or glufosinate (Group 10 [N]), applied to older FTR plants (8-10 leaf) achieved increased phytotoxicity through improved mortality, reduced biomass or fewer seed panicles.
However, the double knock was no better than using paraquat or glufosinate alone when applied to 8 to 10 leaf FTR plants. FTR is not listed on glufosinate labels in Australia but is used to control other weeds in fallow situations at the rate (750 g a.i. per ha) tested in this study. For best results, glufosinate needs to be applied in warm, humid conditions, which is not a common scenario for summer fallow situations.
Rate response (0, 187.5, 375 and 750 g a.i. per ha) to glufosinate applied to large FTR plants.
Are clethodim or haloxyfop suitable alternative herbicides to treat large, glyphosate resistant FTR plants?
In brief: Possibly. Excellent results were achieved in pot trials conducted in an open environment, but will be more difficult to achieve in the field.
The details: Clethodim and haloxyfop were tested on FTR plants at the 24 to 28 leaf stage. Clethodim is registered for use against FTR in a number of summer crops, but without any crop competition many FTR plants survived the registered rate (90 g a.i. per ha), although weed biomass and seed production was severely curtailed.
Haloxyfop efficacy against FTR at this growth stage was 100 per cent at the registered rate of 80 g a.i. per ha.
A combination of these two treatments also resulted in 100 per cent control. The effective use of these two herbicides (both Group 1 [A]) relies on excellent coverage and application when the plants are actively growing. This is difficult to achieve in field conditions, which is why the label recommendations are typically for younger weeds.
A combination of clethodim and haloxyfop can provide good control of large feathertop Rhodes plant and curtail seed production.
These herbicides are known to readily select for resistant biotypes so when applied in a chemical fallow situation (with no competition), it is necessary to target small weeds with robust application rates and to apply a second knock with a contact herbicide, such as paraquat. 
Did you find any new and exciting prospects for controlling mature FTR plants?
In brief: Yes, it seems that there is a truly synergistic effect when isoxaflutole (Group 27 [H]; e.g. Balance) is mixed with paraquat.
The details: Neither of these herbicides provided useful control of FTR at the 40 to 50 leaf stage when applied individually. When mixed together, these herbicides achieved a higher level of weed mortality and prevented panicle production. For example, a tank mixture of isoxaflutole 75 g a.i. per ha, with paraquat 600 g a.i. per ha, resulted in 92 per cent FTR mortality and no panicle production.
Even at a paraquat rate of 300 g a.i. per ha mixed with isoxaflutole 75 g a.i. per ha, only 17 per cent of the large FTR plants survived when the mixture was applied to both the plant and the nearby soil – allowing uptake through both the leaves and the roots.
The benefit of this mixture may be reduced if the weed patch is dense, potentially reducing the amount of the isoxaflutole that reaches the soil. Even the prevention of seed set in large FTR plants is of significant value in managing the seed bank of this invasive weed, as FTR seed remains viable for less than 12 months.
Such a use pattern is not currently specified on product labels, although both products are registered for weed control in fallow situations.  
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Can pulse cover crops tackle multi-resistant ryegrass in irrigated systems?

The best weed control comes from tactics that also bring other benefits to a farming system.
Greg Sefton, principal agronomist with Sefton Agronomics in the Riverina, says multi-resistant annual ryegrass is becoming a major problem in irrigated systems.
Greg Sefton, principal agronomist with Sefton Agronomics in the Riverina, says legume cover cropping is providing effective control of multi-resistant annual ryegrass in irrigated systems.
“Herbicide resistance can move easily through irrigation areas, particularly when the control methods used on the supply channels are limited to just a few herbicides,” he says. “The ryegrass here is generally accepted to have resistance to glyphosate (Group 9 [M]), Group 1 [A] such as clethodim, Group 2 [B] and Group 3 [D], such as trifluralin. Growers are now relying heavily on Group 15 [K] products such as Sakura, and doing their best to rotate out of the problem.”
To regain control, Greg is working with growers to incorporate a multi-purpose fallow crop such as field pea into the system as a winter fallow clean with the added benefit of contributing biological nitrogen into the soil ahead of planting rice or wheat. 
Earlier maturing varieties of field pea provide better weed control options than Kaspa field pea, chickpea and lupin, all of which generally mature later, sometimes after the target weeds have set seed.
“A competitive pulse crop terminated at maximum biomass is an excellent way to reduce weed seed set,” says Greg. “It is a cultural control that also enables the use of some herbicides that are rarely used in our system. Combining the herbicide and cultural methods in the WeedSmart Big 6 is an effective way to keep our cropping options open and to maximise the value of applied water.”

What is the best fit for the legume crop as a winter clean?
In brief: In the Riverina, the optimal place in the rotation is ahead of rice.
The details: Fields selected for rice production are usually bare fallowed for the preceding winter. The aim of the fallow is to control weeds and conserve soil moisture.
Some growers are having success with field pea sown in May as a winter cover crop then terminated for silage or as a brown manure in early September. This fits well with preventing seed set in annual ryegrass, including late germinating plants.
Field pea is a competitive legume and can suppress weed germination and growth when planted in the most competitive configuration possible with minimal soil disturbance and no gaps.
A knockdown treatment of glyphosate (Group 9 [M]), clopyralid (Group 4 [I]) and carfentrazone (Group G [14]) is applied at planting then a mix of pendimethalin (Group 3 [D]), clomazone (Group 13 [Q]) and paraquat (Group 22 [L]) is applied after an irrigation flush to initiate rice germination and prior to rice germination to knockdown both newly emerged barnyard grass (BYG) and persisting ryegrass. This provides a double knock on ryegrass whilst applying a pre-emergent herbicide for barnyard grass in the rice phase.
When implemented once every 4 or 5 years, with a diverse rotation of winter and summer crops in-between, growers can keep a lid on herbicide resistant annual ryegrass populations. 
Field pea is a competitive legume crop that can reduce annual ryegrass germination in the paddock and halt encroachment from the crop borders.
How do you manage weeds on the non-crop areas?
In brief: The same herbicide mix is applied to the whole paddock, including the weeds growing in the check banks.
The details: Weed seed, often carrying herbicide resistance genes, travels easily through irrigation systems and can colonise non-crop areas. Seed from these plants readily infests the cropping areas if not controlled effectively. The control measures used on non-crop zones are often limited to herbicide tactics, so it is important to make sure the herbicide is applied to maximum effect to prevent seed set.
Farm hygiene and physical removal of isolated weeds will also have a positive impact on weed seed production. 
What farming system benefits come with growing a legume cover crop?
In brief: A legume crop grown for biomass rather than grain can improved soil tilth and reduce crusting on some sodic soils. This practice also allows better soil nutrition management and keeps the grower’s options open if the water allocation situation changes.
The details: The field pea crop will fix atmospheric nitrogen and this allows the grower to use 100 to 150 kg/ha less urea to grow the following rice crop without any yield penalty. If there is insufficient irrigation water available for a rice crop, then the fixed nitrogen is still available for a winter crop of canola or wheat.
The phosphorus fertiliser required for rice can be applied when the field pea crop is planted, giving the phosphorus time to become more available in the soil and ready for uptake when the rice is planted.
Field pea is quite drought tolerant, so if irrigation water is not available for rice, the field pea can be grown through to harvest the grain and will usually yield 0.7 t/ha, which can be more profitable than, say, a 1 t/ha drought-affected wheat crop.
Building an integrated farming system based on methods that have multiple benefits is fundamental to staying ahead of weed pressure.
Practical tips for growing field peas as a brown manure crop
Pulses to attack weeds on many fronts

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Can multi-species planting provide effective weed control?

Crop competition is one of the most effective weed control tools available to growers, but some crops simply don’t have a competitive edge.
Dr Andrew Fletcher, a farming systems scientist with CSIRO, says companion planting and intercropping is an option that growers can consider to bolster the competitiveness of an otherwise uncompetitive but valuable crop in the rotation. International research suggests that it can!
Andrew Fletcher, CSIRO farming systems researcher sees potential for multi-species plantings to compete with weeds. Photo: GRDC
“When two or more species are grown together they can occupy ecological niches that might otherwise be taken up by weeds,” he says. “Multi-species plantings have several potential benefits including increased crop yield and improved soil health, but the right combination can also reduce weed biomass by over 50 per cent.”
Multi-species plantings can be quite challenging to integrate into a grain cropping rotation but are more easily used in mixed grain and livestock operations and in intensive pastures for dairy cattle. International research suggests there is a significant untapped opportunity to increase the use of these systems in Australian grain production systems. However, relevant Australian data is scarce and more research is required to understand this untapped potential in Australian systems.    
A mixed-species cover crop can provide multiple soil health benefits, grazing and fodder for livestock and weed control through crop competition and stopping weed seed set.
“Crop competition is a non-herbicide pillar in the WeedSmart Big 6, with the potential to do some serious heavy lifting in terms of weed control,” says Andrew. “Intercropping and companion planting offers a means to bolster the competitiveness of some crops and to keep them in the rotation without risking a weed blow-out.”
What is intercropping, companion planting and mixed-species planting?
In brief: These systems all involve planting two or more crop species together. The combinations are almost limitless.
The details: Intercropping involves planting two or more species together and harvesting the grain of multiple crops. This generally relies on the grain species having different size seed and compatible harvest times.
Companion planting involves two or more species planted together with the intention to harvest grain from one species only after grazing or terminating the other species before seed set.
Sowing a low-growing species like clover between the rows of cereal can compete with weeds in the inter-row area, fix nitrogen and provide the basis of a pasture after the cereal grain is harvested. This is one example of companion planting.
Mixed-species planting is used to describe plantings of several species grown together primarily for the soil health benefits, and that may have potential for grazing and or forage conservation.
How do these systems suppress weed growth?
In brief: These multi-species systems are designed to take up the ecological space that might otherwise present and opportunity for weeds to fill.
The details: Intercropping and companion planting provide additional weed control in situations where one of the species is a relatively poor competitor as a sole crop. By maximising competition, weed growth is suppressed by up to 58 per cent compared to the least competitive species grown on its own. If a competitive crop such as barley is sown in the most competitive configuration possible, there is little additional benefit from adding a second species.
The downside of using this multi-species strategy for weed control is that in-crop herbicide options the choice of herbicides is limited. This is mainly due to the common combinations being a grass crop with a legume or brassica, meaning grass and broadleaf herbicide options can’t be used, except for when one species is terminated. This needs to be factored into decisions around intercropping and companion cropping.  
What are the best-bet combinations for enhanced weed control?
In brief: It depends on the farming system and the other reasons for considering a multi-species planting.
The details: If the aim is to produce grain, the species selected should have easily separated seed. A well-known example is peaola (field pea plus canola). A recent review of historical trials showed that the median yield increase was 31 per cent compared to sole crops of peas and canola, but the weed control effects of peaola in Australia are unquantified.
An effective companion planting combination is wheat undersown with tillage radish and a legume. The broadleaf companions are sprayed out at stem elongation, leaving the cereal to mature through to harvest.
If there is livestock in the farming system, dual purpose combinations such as grazing canola plus vetch and oats can provide excellent weed suppression. This mix could be grazed and then terminated as hay or silage at stem elongation.
Multi-species plantings add a layer of complexity to the farming system, but many growers have taken on the challenge and are reaping the rewards in crop yield, soil health and weed suppression.

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