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Testing for herbicide resistance

With Plant Science Consulting Director and Adelaide University weed science team researcher, Peter Boutsalis.

An application of herbicide may cost thousands of dollars, all of which might be wasted if the weeds being targetted are resistant to the products applied. Testing for herbicide resistance can identify which herbicides will work on your weeds.

Dr Peter Boutsalis said the greatest value in testing for herbicide resistance on your property is to have confidence in the products and application methods you are using.

“Testing takes the guesswork out of the equation and gives farmers baseline information that they can use to monitor changes in the weeds on their farms,” he said.

“If low level resistance is identified early there are many more management options available compared to situations where full blown resistance has taken hold.”

Dr Boutsalis said the over use and over reliance on particular herbicides will unavoidably lead to herbicide resistance developing. “We often hear of farmers applying herbicide even though they are not sure if it will work,” he said.

The $300 to $400 cost of testing is insignificant compared to the cost of wasted herbicide, lost production and the costs of driving down a large seed bank of resistant weeds.

Select dose response

What herbicide resistance tests are available to farmers in Australia?

Short answer: The ‘quick’ test using the whole plant and the ‘seed’ test.

Longer answer: The ‘quick’ test uses plant samples collected on farm and sent to the laboratory. The plants are revived and planted into pots then tested against the required herbicides. The ‘seed’ test requires the collection of ripe seed, which is planted out at the laboratory. After dormancy has been broken and the seedlings have started to grow they are tested for their response to herbicides. Both tests are equally accurate. The ‘quick’ test can not test for resistance to some pre-emergent herbicides, such as trifluralin.

Which is the most common test that farmers use?

Short answer: The seed test.

Longer answer: Collecting seed before or at harvest is the most common method used. The collected seed must be mature, from green to when the seed changes colour. Before harvest collect 30 to 40 ryegrass seedheads or several handfuls of wild oats seed. After harvest it is common to find seedheads still in the paddock or samples of contaminated grain can be sent for analysis.

Wild radish- Eclipse resistance-2

Where is the best place to collect samples?

Short answer: From suspicious or high risk areas.

Longer answer: Herbicide resistance can develop in high risk areas like fencelines or at random through a paddock. Visual observations and changes on the yield monitor in the header can indicate good places to collect seed. If collecting plant samples, look for weeds at the early tillering stage that appear to have ‘escaped’ previous herbicide treatment. Collect 50 to 100 small plants or fewer larger plants. Shake off the soil from the roots, place in a plastic bag and send to the laboratory.

What’s involved in sending samples?

Short answer: Pick, pack, register and ship.

Longer answer: Each sample needs to arrive at the laboratory with suitable identification and instructions. Register the samples online to get a unique sample number and to provide the information required, such as which herbicides you want to test against. Plant Science Consulting and Charles Sturt University both offer commercial herbicide resistance seed testing. Find the details under Point 4 of the 10 Point Plan on the WeedSmart website.



How to ask a WeedSmart question

Ask your questions about the spread of herbicide resistance, or any herbicide resistance management strategy, using this blog or using Twitter @WeedSmartAU.

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How can I be certain that herbicide residues in the soil have fully degraded at planting?

In the course of a chemical fallow there are often several applications of herbicide and some residues may still be present on or near the soil surface when it is time to plant the next crop. In particularly dry years, residues may even carryover from the crop prior to the fallow. NSW Department of Primary Industries soil scientist, Dr Mick Rose, says there has been concern in recent years about the effect these residues may have on soil microbial activity and on the establishment and growth of crops following the fallow, even after the plant back period. Dr Mick Rose, DPI NSW soils researcher, is developing tests and predictive models to support growers in their decisions about crop choice after using residual herbicides. (Photo: GRDC). “Glyphosate has been the most commonly used knockdown herbicide in northern fallows for several decades and more recently growers have been looking to use more diverse programs that include chemicals with residual activity on weeds,” he says. “The increased use of imazapic and diuron have been of most concern to growers when choosing the next crop, particularly after a low rainfall fallow period.” With investment from GRDC, Mick has been working on a project led by Dr Michael Widderick from the Department of Agriculture and Fisheries, Queensland to develop a soil test for imazapic and diuron residues that will indicate damaging residue levels and help growers to decide which crops would be safe to plant in a paddock. “We are determining the threshold levels of residues of these two herbicides at which crop damage is likely for six crops, both winter and summer growing, in a range of soil types,” he says. In earlier work he also looked at the level of glyphosate residue in soils around the country at planting time and the impact these residues have on soil biological processes. “We found that residues of glyphosate were commonly detected in the soil at planting but there was no indication that the herbicide was adversely affecting soil biological activity,” says Mick. “This suggests that the label recommendations are suitable and the proper application of glyphosate in Australia is not posing a threat to soil health.” “For growers to be able to keep using glyphosate they need to implement the WeedSmart Big 6 strategy, including using diverse chemistry in fallows,” he says. “Residual herbicides are a useful tool for growers but there are some gaps in our knowledge about how these herbicides break down in different soils and under different seasonal conditions.” Why not just follow the plant back recommendations on the label? In brief: The label provides the minimum plant back period provided certain environmental conditions are met. There is a possibility of crop injury even though plant back periods are observed. The details: Many factors affect the bioavailability of a herbicide in the soil. For example, even though a clay soil and a sandy soil might have similar residue levels, more herbicide will be available for uptake in the sandy soil. More rain will increase the rate of breakdown, but it is not known exactly how much rain will ensure the specific soil is ‘safe’ to plant into. Another important factor is that many things can contribute to a germination failure. In some situations, residual herbicide may be suspected as the culprit, but can be difficult to either rule it in or out with certainty when diagnosing the reason for a problem at planting. If herbicide residues in the plant tissue can be shown to be phytotoxic, then another, less susceptible crop could be sown into the paddock. Dr Annie Ruttledge, DAF Qld weeds researcher, inspecting chickpea plants growing in soils containing different levels of imazapic and diuron herbicide residue. What effects can herbicide residues have on emerging crops? In brief: The herbicide itself can inhibit germination and growth, or it can exacerbate other factors, such as root disease. The details: At different levels of bioavailability, herbicide residues will have different effects on crop plants. If the herbicide is readily available to the plant, then susceptible crops will take it up from the soil and it can have phytotoxic effects ranging from suppressed vigour to yellowing and potentially plant death. Testing the plant tissue of a struggling crop can show if the leaves contain sufficient herbicide to have caused the observed symptoms. Some herbicide residues in soil can also ‘prune’ plant roots, particularly the fine roots that help access moisture and nutrients. Obviously, if the young plants are struggling to access resources then they will be less vigorous and possibly die. Damaged roots are also more susceptible to water stress, disease and poor nodulation in legumes, making it difficult to determine the initial cause of the problem in the field. If herbicide residues are shown to be the problem then a more tolerant crop can be sown, speeding up the breakdown of the residue and there will be more rainfall events before the next cropping season comes around. Seedling emergence and establishment is being measured for six crops (winter and summer) in the presence of different levels of herbicide. What pre-planting soil tests are being developed to give growers confidence to plant? In brief: The current project is establishing phytotoxicity thresholds for six summer and winter crops in a range of soil types, for two herbicides – imazapic and diuron. The details: By mid-2021 the aim is to have established the thresholds so that soil could be tested pre-plant to determine what crops would be safe to plant. This will give growers confidence to use these herbicides in a diverse strategy to manage weeds like feathertop Rhodes grass in the fallow, while avoiding germination or establishment failures in the following crop. Spray records play an important role in the management of these herbicides and mistakes can easily be made if the spray history for the past several years is not taken into account. In time, growers and their agronomists will gain a better understanding of how these herbicide residues behave in the soils on a particular property and will be able to make herbicide application and crop rotation decisions with more confidence. In another project with the Soil CRC, Mick is developing a predictive model for herbicide breakdown for a wider range of herbicides used in southern and western cropping systems. Until these tests and models become available, the use of an in-field or pot bioassay with a susceptible crop can be helpful in determining potential plant back issues.   Related resources Herbicide residues in soil – the scale and significance (GRDC Update paper) Herbicide residues in soil (GRDC Podcast)
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Never cut the herbicide application rate

Scientific studies have demonstrated that resistance can rapidly evolve in weeds subjected to low doses of herbicide. Some weeds can develop resistance within a few generations. Full rates when mixing herbicides too! When mixing herbicides it is important that each product is still applied at the full label rate to ensure high mortality. Applying different chemicals in one mix can provide an additive advantage. It is important to understand the mode of action of each herbicide on the plant when preparing a herbicide mix. This is just as important for pre-emergent grass weed mixes as it is for post-emergent mixes aimed at broadleaf weed control. ALWAYS READ THE LABEL. Surrounding weed seeds with a combination of pre-emergent herbicides with different modes of action can give a high level of control and help extend the useful life of all the chemicals used. The high level of control must be supported with additional control measures for all survivors. All products with different modes of action must be applied at full label rates for this to be an effective strategy.   Mixing two chemicals with the same mode of action can achieve some additional efficacy, however, the mix should deliver the combined full rate to ensure a lethal dose. The amount of stubble present and crop safety are all important considerations when mixing chemicals. For example, when using a tank mix of Avadex® and trifluralin to control ryegrass in wheat, the rates used will vary depending on the sowing system and level of stubble retention. Be sure to get good advice. Many herbicides on the market are a combination of two or more modes of action within the one product. These products must be applied at the full label rate to be effective. Having dual action does not negate the need to change herbicide products and rotate modes of action. Repeated use of any single strategy will reduce the effectiveness of that strategy over time.  
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Spray well – correct nozzles, adjuvants and water rates

Spray application is a technical field and growers need to make sure their equipment and application techniques are spot-on. The GRDC Spray Application GrowNote provides detailed information and about 80 videos to demonstrate key skills. Prevent spray-drift The focus of spraying herbicide needs to be on doing the job right so the weeds receive the correct dose and die, and this includes reducing the air borne fraction to a bare minimum. Bill Gordon’s 10 Tips for Reducing Spray Drift Choose all products in the tank mix carefully. Understand the product mode of action and coverage requirements. Select (and check) the coarsest spray quality that will provide effective control. Expect that surface temperature inversions will form as sunset approaches and will likely persist overnight and even beyond sunrise on many occasions. DO NOT SPRAY. Use weather forecasts to inform your spray decisions. Only start spraying when the sun is about 20 degrees above the horizon and when the wind speed has been above 4–5 km/hr for more than 20–30 minutes, and clearly blowing away from any adjacent sensitive crops or areas. Set the boom height to achieve a double overlap of the spray patterns. Avoid higher spraying speeds. Leave buffers unsprayed if necessary and come back. Continue to monitor conditions, particularly wind speed, at the site during the spray operation High water rates don’t have to slow you down Some growers are concerned that increasing the water rate when applying herbicide will slow down their spray operation and cost them money. However, the biggest financial loss during spraying usually comes from a failed spray job. To keep your spray operation as time efficient as possible when using more effective and reliable application volumes, you can: Use nurse tanks around the farm to reduce the time spent travelling back to a central re-fill point. Use a larger pump, e.g. 2.5 inch, to make re-filling quicker. Pre-mix the batch while the sprayer is operating. Many mixes can be held in the mixing tank for up to 6 hours. However, wettable granules and suspension concentrates will need agitation to keep them in solution. For pre-emergent herbicides in high stubble situations, carrier volume has a large effect on the level of control achieved. Across four trial sites Dr Borger’s research demonstrated that ryegrass control with trifluralin or Sakura® increased from 53% control when the carrier volume was 30 L/ha to 78% control when the carrier volume was increased to 150 L water/ha in high Water quality and mixing order Water quality is often overlooked as a possible contributor to herbicide failure and can lead to confusion over the herbicide resistance status of weeds on a property. Water should be considered as one of the chemicals in any mix, given that water quality varies markedly depending on its source. Getting the mixing order right is essential for effective spray results. Don’t start mixing until the water quality is right Podcast – Mixing herbicides Adjuvants Sometimes adding an adjuvant is beneficial and sometimes it is detrimental; and there is an art to knowing how to best deploy these additives. When weeds are susceptible to the applied herbicides, the effectiveness of adjuvants generally goes un-noticed. Correctly applied adjuvants can reduce the impact of low level herbicide resistance by helping to maximise the amount of herbicide taken up by the plant.

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